In chemistry labs, automatic delivery pipets or pipettors are used to measure small volumes of 10-1000 ?L (0.010-1.000 mL) accurately and reproducibly. These pipets have disposable plastic tips that hold the preset volume of liquid, and the pipet itself should never be used without a tip in place. The plunger of automatic pipets has two stops; to fill the tip, press the plunger to the first stop and slowly release it to fill the tip with liquid. To deliver the sample, push the plunger fully to the second stop. These pipets are expensive and need to be calibrated before use, and the actual amount of liquid transferred depends on various factors. You can calibrate an automatic pipet by delivering a preset amount of liquid and weighing the flask to determine the exact amount of liquid transferred.
Common questions for this item:
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How do automatic pipettors work? Answer: Automatic pipettors have disposable plastic tips that hold the preset volume of liquid. The plunger of automatic pipettors has two stops, and to fill the tip, press the plunger to the first stop and slowly release it to fill the tip with liquid. To deliver the sample, push the plunger fully to the second stop.
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What is the difference between automatic pipettors and manual pipettes? Answer: Automatic pipettors have disposable plastic tips that hold the preset volume of liquid, while manual pipettes require users to measure and dispense the liquid manually.
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How accurate are automatic pipettors? Answer: Automatic pipettors are very accurate and can measure small volumes of 10-1000 ?L (0.010-1.000 mL) reproducibly.
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How do you calibrate an automatic pipettor? Answer: To calibrate an automatic pipettor, deliver a preset amount of liquid and weigh the flask to determine the exact amount of liquid transferred. If the automatic pipettor needs to be recalibrated, consult your instructor.
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Can you reuse the plastic tips of automatic pipettors? Answer: No, you should not reuse the disposable plastic tips of automatic pipettors. You should use a new tip for each measurement.
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What are some common types of automatic pipettors? Answer: Some common types of automatic pipettors include single-channel pipettors, multi-channel pipettors, and electronic pipettors.
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How do you choose the right size tip for an automatic pipettor? Answer: The tip size should correspond to the volume being measured. You should choose a tip size that is compatible with the pipettor being used.
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Can automatic pipettors be used with corrosive liquids? Answer: Automatic pipettors can be used with corrosive liquids, but you should use tips that are specifically designed for corrosive liquids and dispose of them properly after use.
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How do you store automatic pipettors? Answer: Automatic pipettors should be stored in a clean, dry place, and the plunger should be stored in the upward position to prevent damage.
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How do you clean automatic pipettors? Answer: Automatic pipettors should be cleaned according to the manufacturer's instructions. Typically, the tips should be disposed of after use, and the pipettor body should be wiped down with a mild detergent or disinfectant.